Description
Intended Uses: The Human Granzyme B ELISA is to be used for the in-vitro quantitative determination of Granzyme B in human serum, plasma, buffered solutions or cell culture medium.
Principle of the Assay: The Granzyme B Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for Granzyme B has been coated onto the wells of the microtiter strips provided. Samples, including standards of known Granzyme B concentrations and unknowns are pipetted into these wells. During the first incubation, the Granzyme B antigen and a biotinylated monoclonal antibody specific for Granzyme B are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution which is acting on the bound enzyme is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of Granzyme B present in the samples